Not if you record a track later in the song. Like a lead solo or something where you record from a marker point later in the time line. What I do is bounce the that track to another so that when I export to DAW the bounced track is the same length as the others. I also use the "clean-up" function to delete track files that have been "recorded over". It's kind of a pain but still worth it to me because I mix in the box. I only use my 24 to track and leave all effects and processing to the DAW.