If you look at the configuration in your recording program, you should see several different input sources, such as FW410 Analog Left, Analog Right, Analog Stereo, S/PDIF Left, S/PDIF Right, S/PDIF Stereo, etc... If you want to record several tracks at once, then make sure that each instrument or mic is coming in on it's own input plug, and then just set the left and right input sources to different tracks in the recording program.