Yes, as long as you have an open pci slot. I have the Darla and Soundblaster gold 32, I use the Darla for audio recording and the soundblaster for midi and the synthesizer. Make sure you go to websites and get all updated drivers for both cards. You may have to disable your original card while using the darla and soundblaster to free up interupters, I did and am using my soundblaster as my default card. If the darla and soundblaster cards are your only cards, you should be good to go. I have Cooledit pro 1.2a, cakewalk, and jammer pro 4.0. What a great time I have using both cards together. Good Luck
